| Customization: | Available |
|---|---|
| Type: | IVD Reagent |
| Model No.: | MPV01 |
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*Monkeypox is a zoonotic viral disease caused by the monkeypox virus. It occurs primarily in tropical rainforest areas
of Central and West Africa and is occasionally exported to other regions.
1. Reagent Preparation(performed at "reagent preparation region")
1.1 Take each component from the diagnostic kit and place them at room temperature. Allow the reagents to equilibrate at room temperature, then vortex each of them respectively for later use.
1.2 According to the number of test specimens, MPV Positive Control, MPVNegative Control, and Internal control (IC), pipette appropriate quantity of MPVPCR Mix andMPVEnzymeMix ( MPV PCR Mix 15 μl/test +MPV EnzymeMix 4 μl/test +Internal control (IC) 1 μl/test), mix them thoroughly to make a PCR-Mastermix, centrifuge instantaneously for later use.1.3 Transfer the above-prepared reagents to the"specimen processing region" for later use.
2. Processing and loading of specimens (performed in the "specimen processing region")
2.1 This diagnostic kit does not include Viral RNA&DNA Extraction Kit. It is recommended to use Viral RNA&DNA ExtractionKit produced by Changsha Renji Medical Equipment Co., Ltd. to extract viral DNA. The specific operation is in accordance with its instructions.
2.2 Add 20μl PCR-Mastermix into PCR reaction tube with 5μl above processed sample, MPV Positive Control, and MPV NegativeControl, and cap the tube. Carry out fluorescence quantitative PCR detection on fluorescence PCR instrument.
3.PCRAmplification (performed at "nucleic acidamplificationarea")
3.1 Place PCR reaction tubes into the specimen wells of the amplification equipment. SetupMPVPositive Control, MPV NegativeControl, and specimens to be tested in the corresponding sequence and input specimen name.
3.2 Set cycle parameters according to the following table for PCR amplification.
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