| Customization: | Available |
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| Type: | IVD Reagent |
| Nucleic Acid Type: | DNA |
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Monekypox Virus Real Time PCR Kit - a detection tool to combat the potential spread among people and animals. CE marked.
What is the monkeypox virus?
Monkeypox was first discovered in 1958 when two outbreaks of a pox-like disease occurred in colonies of monkeys kept for research, hence the name 'monkeypox.' The first human case of monkeypox was recorded in 1970 in the Democratic Republic of the Congo (DRC) during a period of intensified effort to eliminate smallpox. Since then, monkeypox has been reported in several other central and western African countries: Cameroon, Central African Republic, Cote d'Ivoire, the Democratic Republic of the Congo, Gabon, Liberia, Nigeria, Republic of the Congo, and Sierra Leone. The majority of infections are in the Democratic Republic of the Congo.
Monkeypox cases in people have occurred outside Africa linked to international travel or imported animals, including patients in the United States and Israel, Singapore, and the United Kingdom.
The natural reservoir of monkeypox remains unknown. However, African rodents and non-human primates (like monkeys) may harbor the virus and infect people.
How many countries have been infected?
According to WHO, "as of 21 May, 13:00, 92 laboratory-confirmed cases and 28 suspected monkeypox cases with ongoing investigations have been reported to WHO from 12 Member States that are not endemic monkeypox virus across three WHO regions (Table 1, Figure 1). No associated deaths have been reported to date."
• Rapid - Completes the entire process from extraction to amplification within a minimum of 45 minutes
• Flexible - Three specifications fulfill different demands
• Accurate - Assay sensitivity of 200 copies/mL
• Specimen requirement - Serum or plasma, vesicular or pustular lesion fluid
• 50 tests/kit & 200 tests/kit
• Specimen requirement: Serum or plasma, vesicular or pustular lesion fluid
• Applicable Instruments: MA-6000, ABI series, Bio-Rad series, Roche LightCycler R480, Cepheid SmartCycler, Rotor-Gene series and other multi-channel real-time quantitative PCR instruments.
1. Reagent Preparation(performed at "reagent preparation region")
1.1 Take out each componentsfrom the diagnostic kitand placethemat room temperature. Allow thereagents to equilibrateatroom temperature, then vortex each of themrespectively forlater use.
1.2 According to thequantityof test specimens, MPV Positive Control, MPVNegative Control,and Internal control (IC), pipetteappropriate quantity of MPVPCR Mix andMPVEnzymeMix ( MPV PCR Mix 15 μl/test +MPV EnzymeMix 4 μl/test +Internal control (IC) 1 μl/test), mix them thoroughlytomake a PCR-Mastermix, centrifuge itinstantaneously forlater use.
1.3 Transfer the above-prepared reagents to the"specimen processing region" for later use.
2. Processing and loading ofspecimens (performed at "specimenprocessing region")
2.1 Thisdiagnostic kit does not include Viral RNA&DNA Extraction Kit. Itisrecommended to use Viral RNA&DNA ExtractionKit produced by Changsha Renji Medical Equipment Co., Ltd. to extract viral DNA. The specific operation is in accordancewith its instructions.
2.2 Add 20μl PCR-Mastermix into PCR reaction tube with 5μl above processed sample, MPV Positive Control and MPV NegativeControl, and cap the tube. Carry outfluorescence quantitativePCR detection on fluorescencePCR instrument.
3.PCR Amplification (performed at "nucleic acidamplificationarea")
3.1 Place PCRreaction tubes into thespecimenwells of the amplification equipment. SetupMPVPositive Control, MPV NegativeControl and specimens to be tested in the corresponding sequence and input specimen name.
3.2 Set cycle parameters according to the following table for PCR amplification.
Changsha Renji Medical Equipment Co.,Ltd is a medical supply company specilizing in products series, such as disposable virus specimen collection sampler and Saliva collection kits for PCR test. Vacuum blood collection tubes and other medical consumables. We are direct manufacturer based on Changsha City Hunan Province. And many of our staffs are gene experts and professional doctors who have plenty of experience in this industry. Our products have been approved by many certificates. Such as CE, SGS,ISO and so on.
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