| Customization: | Available |
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| Type: | IVD Reagent |
| Nucleic Acid Type: | DNA |
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Cat.No.
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MPV01 |
| Description | Monkeypox Virus Real Time PCR Kit is used for the detection of monkeypox Virus in serum or lesion exudate samples by using real time PCR systems. Monkeypox virus (MPV) is a double-stranded DNA, zoonotic virus and a species of the genus Orthopoxvirus in the family Poxviridae. It is one of the human orthopoxviruses that includes variola (VARV), cowpox (CPX), and vaccinia (VACV) viruses. The kit contains a specific ready-to-use system for the detection of the monkeypox Virus. Fluorescence is emitted and measured by the real time systems' optical unit during the PCR. |
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Nucleic Acid Type
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DNA |
| Species | virus |
| Storage | • Store at -20°C • Repeated thawing and freezing (>66x) should be avoided • Cool all reagents during the working steps |
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Instrument
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The diagnostic kit is applicable to MA-6000, ABI series, Bio-Rad series, Roche LightCycler R480, Cepheid SmartCycler, Rotor-Gene series and other multi-channel real-time quantitative PCR instruments. |
1. Reagent Preparation(performed at "reagent preparation region")
1.1 Take out each componentsfrom the diagnostic kitand placethemat room temperature. Allow thereagents to equilibrateatroom temperature, then vortex each of themrespectively forlater use.
1.2 According to thequantityof test specimens, MPV Positive Control, MPVNegative Control,and Internal control (IC), pipetteappropriate quantity of MPVPCR Mix andMPVEnzymeMix ( MPV PCR Mix 15 μl/test +MPV EnzymeMix 4 μl/test +Internal control (IC) 1 μl/test), mix them thoroughlytomake a PCR-Mastermix, centrifuge itinstantaneously forlater use.
1.3 Transfer the above-prepared reagents to the"specimen processing region" for later use.
2. Processing and loading ofspecimens (performed at "specimenprocessing region")
2.1 Thisdiagnostic kit does not include Viral RNA&DNA Extraction Kit. Itisrecommended to use Viral RNA&DNA ExtractionKit produced by Changsha Renji Medical Equipment Co., Ltd. to extract viral DNA. The specific operation is in accordancewith its instructions.
2.2 Add 20μl PCR-Mastermix into PCR reaction tube with 5μl above processed sample, MPV Positive Control and MPV NegativeControl, and cap the tube. Carry outfluorescence quantitativePCR detection on fluorescencePCR instrument.
3.PCRAmplification (performed at "nucleic acidamplificationarea")
3.1 Place PCRreaction tubes into thespecimenwells of the amplification equipment. SetupMPVPositive Control, MPV NegativeControl and specimens to be tested in the corresponding sequence and input specimen name.
3.2 Set cycle parameters according to the following table for PCR amplification.
Changsha Renji Medical Equipment Co.,Ltd is a medical supply company specilizing in products series, such as disposable virus specimen collection sampler and Saliva collection kits for PCR test. Vacuum blood collection tubes and other medical consumables. We are direct manufacturer based on Changsha City Hunan Province. And many of our staffs are gene experts and professional doctors who have plenty of experience in this industry. Our products have been approved by many certificates. Such as CE, SGS,ISO and so on.
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